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Szuchet, Sara

One or more keywords matched the following properties of Szuchet, Sara

Property	Value
overview	The long-term goal of my laboratory is to decipher the etiology and pathogenesis of multiple sclerosis (MS) – a CNS demyelinating and neurodegenerative disease that cripples the young and bright. With the firm belief that in order to decrypt the breakdown of myelin in MS, we have to understand how it is formed, our current efforts are centered on two major projects. One, defining the molecular mechanism of myelin sheath formation by following the synthesis of myelin membranes (MyMs), their packaging into myelinophore organelles (MFOs) and transportation along an oligodendrocyte process using live imaging of embryonic avian optic nerve at different stages of development; purifying and characterizing the contents of MFOs. Two, testing the hypothesis that ectosomes are the conveyors of signals that coordinate and orchestrate the modular structure of the myelinated axon. We are developing technologies to obtain pure cell-specific ectosomes by molecularly color-code them. We are also customizing a microfluidic platform suitable to integrate multiple processes in a single lab-on-a-chip format. We plan to establish unique EM grid compatible bi-chamber for OLG-neuron cultures and capture the formation of cell-specific ectosomes using correlative light and electron microscopy and phase contrast cryo-electron tomography. We will assemble tomograms of the initial OLG-neuron encounter so that we can structurally model the interface in a 3D space.

Property

Value

overview

The long-term goal of my laboratory is to decipher the etiology and pathogenesis of multiple sclerosis (MS) – a CNS demyelinating and neurodegenerative disease that cripples the young and bright. With the firm belief that in order to decrypt the breakdown of myelin in MS, we have to understand how it is formed, our current efforts are centered on two major projects. One, defining the molecular mechanism of myelin sheath formation by following the synthesis of myelin membranes (MyMs), their packaging into myelinophore organelles (MFOs) and transportation along an oligodendrocyte process using live imaging of embryonic avian optic nerve at different stages of development; purifying and characterizing the contents of MFOs. Two, testing the hypothesis that ectosomes are the conveyors of signals that coordinate and orchestrate the modular structure of the myelinated axon. We are developing technologies to obtain pure cell-specific ectosomes by molecularly color-code them. We are also customizing a microfluidic platform suitable to integrate multiple processes in a single lab-on-a-chip format. We plan to establish unique EM grid compatible bi-chamber for OLG-neuron cultures and capture the formation of cell-specific ectosomes using correlative light and electron microscopy and phase contrast cryo-electron tomography. We will assemble tomograms of the initial OLG-neuron encounter so that we can structurally model the interface in a 3D space.

One or more keywords matched the following items that are connected to Szuchet, Sara

Item Type	Name
Concept	Antigen-Antibody Complex
Concept	Antigens, Viral
Concept	Adenylyl Cyclases
Concept	Cyclic AMP
Concept	Amino Acid Sequence
Concept	Cerebral Cortex
Concept	Aminopyridines
Concept	Animals
Concept	Chromatography, DEAE-Cellulose
Concept	Epitopes
Concept	Antibodies, Monoclonal
Concept	Collodion
Concept	Antigens, Bacterial
Concept	Base Sequence
Concept	Autoradiography
Concept	Cattle
Concept	Cell Division
Concept	Cell Separation
Concept	Evaluation Studies as Topic
Concept	Centrifugation, Density Gradient
Concept	Cesium
Concept	Chick Embryo
Concept	Chromatography, Thin Layer
Concept	Coagulants
Concept	Cholesterol
Concept	Chromatography, Ion Exchange
Concept	Collagen
Concept	Culture Media
Concept	Cysteine
Concept	Cysteine Endopeptidases
Concept	Demyelinating Diseases
Concept	Dogs
Concept	Electrophoresis, Polyacrylamide Gel
Concept	Freeze Fracturing
Concept	Galactose
Concept	Glycerol
Concept	Heparitin Sulfate
Concept	Electrophysiology
Concept	Hybridomas
Concept	Diglycerides
Concept	Chondroitin Lyases
Concept	Colforsin
Concept	Ferritins
Concept	Fibrinogen
Concept	Interferons
Concept	Electric Conductivity
Concept	Electrophoresis
Concept	Enzyme Activation
Concept	Models, Chemical
Concept	Galactosylceramides
Concept	Gene Expression Regulation
Concept	Myelin Proteins
Concept	Glycolipids
Concept	Glial Fibrillary Acidic Protein
Concept	Fibronectins
Concept	Fructose-Bisphosphate Aldolase
Concept	Nucleic Acid Hybridization
Concept	Glycosphingolipids
Concept	Hemostatics
Concept	Hydrogen-Ion Concentration
Concept	Immune Sera
Concept	Myelin Basic Protein
Concept	Fluorescent Antibody Technique
Concept	Gene Amplification
Concept	Lactoferrin
Concept	Membrane Glycoproteins
Concept	Hydrolysis
Concept	Mice, Inbred Strains
Concept	Phospholipids
Concept	Mice, Neurologic Mutants
Concept	Mice, Transgenic
Concept	Polylysine
Concept	Extracellular Matrix
Concept	Immunohistochemistry
Concept	Mice, Inbred BALB C
Concept	Mice, Inbred C57BL
Concept	Neutrophils
Concept	Laminin
Concept	Phosphatidylinositols
Concept	Ovalbumin
Concept	Membrane Lipids
Concept	Microscopy, Electron
Concept	Embryonic and Fetal Development
Concept	Polymers
Concept	Polysaccharide-Lyases
Concept	Potassium
Concept	Potassium Radioisotopes
Concept	Protein Conformation
Concept	Proteoglycans
Concept	Gangliosides
Concept	Receptors, Fc
Concept	Rabbits
Concept	Receptors, Cell Surface
Concept	Rosette Formation
Concept	Sodium Dodecyl Sulfate
Concept	Potassium Channels
Concept	Sodium
Concept	Heparin
Concept	Sheep
Concept	Humans
Concept	Sulfoglycosphingolipids
Concept	Sulfur Radioisotopes
Concept	Tritium
Concept	Intracellular Membranes
Concept	Tumor Necrosis Factor-alpha
Concept	Mathematics
Concept	Peanut Agglutinin
Concept	Visna-maedi virus
Concept	4-Aminopyridine
Concept	Cell Adhesion Molecules
Concept	Up-Regulation
Concept	2',3'-Cyclic-Nucleotide Phosphodiesterases
Concept	Multiple Sclerosis
Concept	Cell Adhesion Molecules, Neuronal
Concept	Rats, Sprague-Dawley
Concept	Neuromuscular Depolarizing Agents
Concept	Microscopy, Immunoelectron
Concept	Sheep, Domestic
Concept	Methionine
Concept	Methods
Concept	tau Proteins
Concept	Myelin Sheath
Concept	Myosins
Concept	Nerve Regeneration
Concept	Arachidonic Acid
Concept	Receptors, Tumor Necrosis Factor
Concept	Cell Transplantation
Concept	DNA, Complementary
Concept	Myelin Proteolipid Protein
Concept	Myelin-Associated Glycoprotein
Concept	Vitronectin
Concept	Tetraethylammonium
Concept	Heparan Sulfate Proteoglycans
Concept	Fluorescent Antibody Technique, Indirect
Concept	In Situ Hybridization, Fluorescence
Concept	Protein Structure, Secondary
Concept	Microarray Analysis
Concept	Gene Expression Profiling
Concept	Type C Phospholipases
Concept	Phorbol Esters
Concept	Phosphoric Monoester Hydrolases
Concept	Plasmalogens
Concept	Mice
Concept	Psychosine
Concept	RNA, Messenger
Concept	Stochastic Processes
Concept	Clusterin
Concept	Rats
Concept	Trypsin
Concept	Immunoblotting
Concept	Blotting, Western
Concept	Tetradecanoylphorbol Acetate
Concept	Blotting, Southern
Concept	Blotting, Northern
Concept	GTP-Binding Proteins
Concept	Cell Lineage
Concept	T-Lymphocytes, Regulatory
Concept	Antibodies
Concept	Axons
Concept	Binding, Competitive
Concept	Cell Survival
Concept	Cloning, Molecular
Concept	Fibrinolysin
Concept	Ganglia, Spinal
Concept	Horses
Concept	Kinetics
Concept	Lactoglobulins
Concept	Membrane Potentials
Concept	Microscopy, Fluorescence
Concept	Phosphoric Diester Hydrolases
Concept	Plasminogen
Concept	Recombinant Fusion Proteins
Concept	Sphingosine
Concept	1-Methyl-3-isobutylxanthine
Concept	Thrombolytic Therapy
Concept	Chondroitin ABC Lyase
Academic Article	Myelination by mature ovine oligodendrocytes in vivo and in vitro: evidence that different steps in the myelination process are independently controlled.
Academic Article	Oligodendrocyte proteoglycans: modulation by cell-substratum adhesion.
Academic Article	Three different thyroid hormone receptor isoforms are detected in a pure culture of ovine oligodendrocytes.
Academic Article	Tumor necrosis factor inhibits K+ current expression in cultured oligodendrocytes.
Academic Article	Oligodendrocytes express an alpha/beta-interferon-susceptible Mx gene: molecular characterization of the encoded protein.
Academic Article	Reactive astrocytes upregulate one or more gene products that are recognized by monoclonal antibody H.
Academic Article	Adhesion-induced differentiation of oligodendrocytes signals the synthesis and polarization of heparan sulfate proteoglycans and sulfated glycoproteins.
Academic Article	NOVOcan: a molecular link among selected glial cells.
Academic Article	Further studies with human plasminogen.
Academic Article	The molecular weight of bovine lactoferrin.
Academic Article	Intracellular messengers. Influence of oligodendrocyte substratum adhesion.
Academic Article	Channel-mediated and carrier-mediated uptake of K+ into cultured ovine oligodendrocytes.
Academic Article	Expression and modulation of K+ currents in oligodendrocytes: possible role in myelinogenesis.
Academic Article	The genetic signature of perineuronal oligodendrocytes reveals their unique phenotype.
Academic Article	Forskolin and phorbol esters decrease the same K+ conductance in cultured oligodendrocytes.
Academic Article	Lipid and glycolipid metabolism of cultured astrocytes: a time course study.
Academic Article	Cultured oligodendrocytes. A role for cell-substratum interaction in phenotypic expression.
Academic Article	Maintenance of isolated oligodendrocytes in long-term culture.
Academic Article	Cell-cell interactions of isolated and cultured oligodendrocytes: formation of linear occluding junctions and expression of peculiar intramembrane particles.
Academic Article	Oligodendrocyte adhesion activates protein kinase C-mediated phosphorylation of myelin basic protein.
Academic Article	Plasma membrane of cultured oligodendrocytes: III. Relatedness to myelin.
Academic Article	Signal transduction pathways in oligodendrocytes: role of tumor necrosis factor-alpha.
Academic Article	Detection of visna virus antigens and RNA in glial cells in foci of demyelination.
Academic Article	Myelin palingenesis. 2. Immunocytochemical localization of myelin/oligodendrocyte glycolipids in multilamellar structures.
Academic Article	Equilibrium sedimentation of proteins in acid solutions. Dissociation of aldolase by aqueous acetic acid.
Academic Article	An in-vitro approach to the study of oligodendrocytes and their involvement in multiple sclerosis.
Academic Article	A monoclonal antibody against human T suppressor lymphocytes binds specifically to the surface of cultured oligodendrocytes.
Academic Article	Characterization of a subset of oligodendrocytes separated on the basis of selective adherence properties.
Academic Article	Effect of purification procedures on the self-association of myosin at high ionic strength.
Academic Article	Equilibrium centrifugation of proteins in acidic solutions. Beta-lactoglobulin A in aqueous acetic, propionic, and butyric acids.
Academic Article	Oligodendrocyte-substratum adhesion activates the synthesis of specific lipid species involved in cell signaling.
Academic Article	Synthesis of gangliosides by cultured oligodendrocytes.
Academic Article	Depolarizing agents and tumor necrosis factor-alpha modulate protein phosphorylation in oligodendrocytes.
Academic Article	Oligodendrocyte substratum adhesion modulates expression of adenylate cyclase-linked receptors.
Academic Article	Plasma membrane of cultured oligodendrocytes: I. Isolation, purification, and initial characterization.
Academic Article	Plasma membrane of cultured oligodendrocytes: II. Possible structural and functional domains.
Academic Article	A monoclonal antibody that binds to both astrocytes and myelin sheaths.
Academic Article	GRASP: a novel heparin-binding serum glycoprotein that mediates oligodendrocyte-substratum adhesion.
Academic Article	Myelin palingenesis. 1. Electron microscopical localization of myelin/oligodendrocyte proteins in multilamellar structures by the immunogold method.
Academic Article	Phosphorylation of myelin basic protein in intact oligodendrocytes: inhibition by galactosylsphingosine and cyclic AMP.
Academic Article	A peanut agglutinin binding glycoprotein in CNS myelin and oligodendrocytes.
Academic Article	CNS myelin sheath is stochastically built by homotypic fusion of myelin membranes within the bounds of an oligodendrocyte process.
Concept	2',3'-Cyclic Nucleotide 3'-Phosphodiesterase
Concept	Myxovirus Resistance Proteins

Search Criteria

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Cyclic AMP
Phosphodiesterases